Cooperative CTCF-nucleosome oligomerization stabilizes chromatin loop anchors
Manuel Osorio‐Valeriano, Alexander C. Stone, Masahiro Nagano, Bonnie G. Su, Laura Caccianini, Anders S. Hansen, Lucas Farnung, Seychelle M. Vos
CTCF dimerization on stacked nucleosomes is resolved by cryo-EM through a ZnF6-7-8 interface. Disrupting it in mouse stem cells weakens CTCF-cohesin loops more than insulation and impairs germline differentiation.
bioRxiv · Sep 12, 2026 · PreprintMolecular Biology✦ In-depth digest