Lucas Farnung

The paper reveals that RPA activates TMEJ by facilitating ssDNA hand-off to Polθ, which initiates microhomology search for DNA repair.

CTCF dimerization on stacked nucleosomes is resolved by cryo-EM through a ZnF6-7-8 interface. Disrupting it in mouse stem cells weakens CTCF-cohesin loops more than insulation and impairs germline differentiation.

DNAsight automates AFM image analysis of chromatin organization, revealing protein-specific structural signatures and nucleosome spacing distributions.

This study identifies IWS1 as a modular scaffold that organizes the transcription elongation complex through its disordered region, revealing its role in recruitment and stimulation of elongation.

The paper shows CTCF controls chromatin boundary permeability through a dynamic conformational ensemble, not static occupancy, influencing cohesin capture probabilities.

This study shows that the catalytic pocket of Clr4 (Suv39h) acts as a receptor for Cul4-dependent H3K14 ubiquitination, which regulates Clr4's methylation activity on H3K9.

This study presents cryo-EM structures of the human replisome interacting with a nucleosome, revealing DNA unwrapping and nucleosome disassembly mechanisms during replication.

The paper investigates the role of STK19 in transcription-coupled DNA repair by showing it positions TFIIH for repair in a cell-free system.

The paper uses AlphaFold-Multimer to screen human proteins for nucleosome acidic patch binding, identifies SHPRH as a novel binder, and determines its structure.

The paper reports the cryo-EM structure of the complete S. cerevisiae Rpd3S-nucleosome complex, revealing its subunit arrangement and substrate specificity mechanisms.

The paper reports the cryo-EM structure of CHD4 bound to a nucleosome, explaining how mutations in CHD4 contribute to cancer and intellectual disability.

The paper investigates how transcription factors interact with nucleosomal DNA, finding that most have less access to it than free DNA and that nucleosome structure affects motif positioning and orientation.