Molecular BiologyPLoS ONEPeer-reviewed
High-throughput yeast two-hybrid library screening using next generation sequencing
Marie‐Laure Erffelinck, Bianca Ribeiro, Marı́a Perassolo…·Alain Goossens
The paper introduces Y2H-seq, a high-throughput yeast two-hybrid method using NGS to identify protein-protein interactions without prior ORF knowledge, improving sensitivity and reducing false positives.
51 citations