Laurens Pauwels

BackBone Builder (B3): A modular Golden Gate standard with a compatible Agrobacterium parts library

The paper introduces B3, a modular Golden Gate system for assembling Agrobacterium binary vector backbones, enabling efficient and flexible construction of over 370,000 possible constructs.

Soil-based disease bioassay for the study of rhizogenic Agrobacterium-tomato interactions

The paper develops a soil-based bioassay to study rhizogenic Agrobacterium-tomato interactions, showing it can assess disease symptoms and differentiate virulence among strains.

Gene regulatory network analysis of somatic embryogenesis identifies morphogenic genes that increase maize transformation frequency

The paper identifies morphogenic genes that enhance maize transformation frequency by analyzing gene regulatory networks in induced somatic embryos.

BREEDIT: A novel multiplex genome editing strategy to improve complex quantitative traits in maize (Zea mays L.)

BREEDIT improves complex traits in maize by combining CRISPR/Cas9 gene editing with crossing schemes to generate mutants with enhanced growth characteristics.

High-throughput yeast two-hybrid library screening using next generation sequencing

The paper introduces Y2H-seq, a high-throughput yeast two-hybrid method using NGS to identify protein-protein interactions without prior ORF knowledge, improving sensitivity and reducing false positives.