This study identifies genetic interactions among tRNA modification genes in E. coli K12, revealing synthetic lethal pairs and condition-dependent phenotypes.

3 citations

E. coliexperimental assay

𝕏 PostBluesky

Molecular BiologyNucleic Acids ResearchPeer-reviewedMay 23, 2020

Nick-seq for single-nucleotide resolution genomic maps of DNA modifications and damage

Bo Cao, Xiaolin Wu, Jieliang Zhou…·Peter C. Dedon

The paper introduces Nick-seq, a method for single-nucleotide resolution mapping of DNA modifications and damage, demonstrating its application to single-strand-breaks, phosphorothioate modifications, and DNA oxidation.

67 citations

DNA methylationexperimental assay

𝕏 PostBluesky

Molecular BiologybioRxivPreprintNov 26, 2019

Sequencing-based quantitative mapping of the cellular small RNA landscape

Jennifer F. Hu, Daniel Yim, Sabrina M. Huber…·Bo Cao

The paper introduces AQRNA-seq, a method for accurate small RNA quantification, and demonstrates its application to bacterial tRNA pools revealing copy number variation and modification patterns.

4 citations

bacteria (other)experimental assay

𝕏 PostBluesky