Valérie de Crécy‐Lagard

Identification of a putative RocS homolog through phenotypic profiling of uncharacterized essential genes in Streptococcus mutans

This study identifies SMU_393 as a homolog of RocS in S. mutans through phenotypic profiling of essential genes, revealing its role in chromosome segregation and cell cycle control.

The Escherichia coli Radical SAM Enzyme YhcC Replaces MnmC in mnm5s2U tRNA Modification During Anaerobic Growth

This study identifies YhcC as an anaerobic alternative to MnmC for demodifying cmnm5s2U to nm5s2U in E. coli tRNA.

Functional Divergence and Conservation in the QueC Protein Family (PF06508): From tRNA Modification to Anti-Phage Defense

This study maps the functional diversification of the QueC protein family, identifying residues critical for tRNA modification and characterizing its repurposing in anti-phage defense and other biochemical pathways.

Integrating targeted genome mining and structure-guided modeling reveals unexplored 7-deazapurine-containing pathways

The paper integrates genome mining and structure-guided modeling to discover new 7-deazapurine-containing biosynthetic gene clusters and their chemical functions.

tRNA Modification Landscapes in Streptococci: Shared Losses and Clade-Specific Adaptations

This study maps tRNA modifications in Streptococcus mutans and S. pneumoniae, revealing shared losses and clade-specific adaptations in modification genes and metabolic strategies.

Moraxella catarrhalis HemW is a Heme-binding Radical SAM Enzyme

This study characterizes McHemW, a heme-binding radical SAM enzyme in Moraxella catarrhalis, showing it binds heme and contains an active [4Fe-4S] cluster, suggesting a role in heme transport.

Limitations of Current Machine-Learning Models in Predicting Enzymatic Functions for Uncharacterized Proteins

The paper evaluates machine-learning models' ability to predict enzymatic functions for uncharacterized proteins and finds they fail to integrate scientific reasoning and make logic errors.

tRNA modification profiling reveals epitranscriptome regulatory networks in Pseudomonas aeruginosa

The paper profiles tRNA modifications in Pseudomonas aeruginosa to uncover regulatory networks and validate gene annotations.

The oncogene SLC35F2 is a high-specificity transporter for the micronutrients queuine and queuosine

This paper identifies SLC35F2 as a high-specificity transporter for queuine and queuosine in multiple species and shows its role in regulating intracellular levels of these micronutrients.

Targeted genome mining with GATOR-GC maps the evolutionary landscape of biosynthetic diversity

The paper introduces GATOR-GC, a genome mining tool that identifies diverse gene clusters and maps their evolutionary patterns, revealing previously uncharacterized biosynthetic functions.

Synergistic effects of tRNA modification defects in Escherichia coli K12.

This study identifies genetic interactions among tRNA modification genes in E. coli K12, revealing synthetic lethal pairs and condition-dependent phenotypes.

Four additional natural 7-deazaguanine derivatives in phages and how to make them

The paper identifies four new 7-deazaguanine derivatives in phages and characterizes enzymes involved in their biosynthesis and insertion into DNA.

Sequencing-based quantitative mapping of the cellular small RNA landscape

The paper introduces AQRNA-seq, a method for accurate small RNA quantification, and demonstrates its application to bacterial tRNA pools revealing copy number variation and modification patterns.