Michael S. DeMott

Harmonized nucleoside mass spectrometry enables reproducible cross-platform RNA modification quantification

The paper benchmarks cross-platform RNA modification quantification using LC-MS/MS and finds that harmonized workflows and SILIS normalization improve reproducibility across different instrument types.

Human 28S rRNA analysed by state-of-the-art oligonucleotide mass spectrometry: benchmarking current capabilities and a call to action for MS-Seq

The paper benchmarks MS-Seq for human 28S rRNA analysis, showing reproducible results across platforms but highlighting gaps in coverage and ambiguity.

Standalone nanopore sequencing for foodborne pathogen surveillance: a large-scale evaluation and quality control framework

This paper evaluates standalone Oxford Nanopore sequencing for foodborne pathogen surveillance, showing it can achieve high accuracy with quality control tools like alpaqa.

Phosphorothioate DNA modification by BREX Type 4 systems in the human gut microbiome

The paper identifies new phosphorothioate DNA modification systems in the human gut microbiome using genomic and mass spectrometric analysis.

Distinct age- and pathology-dependent epitranscriptome and translational dysfunction in a tauopathy mouse model

This study investigates age- and pathology-dependent changes in the epitranscriptome and translational dysfunction in a tauopathy mouse model, revealing significant alterations in tRNA biology and codon usage patterns.

Temporal dynamics and metagenomics of phosphorothioate epigenomes in the human gut microbiome

The paper characterizes phosphorothioate (PT) modifications in the human gut microbiome using metagenomics and mass spectrometry, revealing temporal dynamics and species-specific patterns.

Synergistic effects of tRNA modification defects in Escherichia coli K12.

This study identifies genetic interactions among tRNA modification genes in E. coli K12, revealing synthetic lethal pairs and condition-dependent phenotypes.

PT-seq: A method for metagenomic analysis of phosphorothioate epigenetics in complex microbial communities

PT-seq is a high-sensitivity method for mapping phosphorothioate DNA modifications in complex microbial communities and metagenomic identification of PT-containing microbes.

Nick-seq for single-nucleotide resolution genomic maps of DNA modifications and damage

The paper introduces Nick-seq, a method for single-nucleotide resolution mapping of DNA modifications and damage, demonstrating its application to single-strand-breaks, phosphorothioate modifications, and DNA oxidation.

Sequencing-based quantitative mapping of the cellular small RNA landscape

The paper introduces AQRNA-seq, a method for accurate small RNA quantification, and demonstrates its application to bacterial tRNA pools revealing copy number variation and modification patterns.