Bright Probes, Blurred Metabolism: Navigating Fluorescent Protein Cross-Excitation in NADH FLIM
Janielle Cuala, Oscar Alberto de la Fuente, Lynne Cherchia, Yuanzhong Pan, Tvisha Singh, Chris Deng, Andrea Velazquez, Kate White, Senta K. Georgia, Steve Kay, Scott E. Fraser, Falk Schneider
This paper evaluates cross-talk between fluorescent proteins and NADH FLIM, identifying compatible FPs and providing a pipeline to manage cross-excitation in metabolic imaging.
bioRxiv · May 29, 2026 · PreprintBiophysics