Alistair J. McCormick

This study characterizes the pyrenoid structure and molecular components of Chlorella sorokiniana, revealing conserved inorganic carbon delivery machinery and novel proteins involved in pyrenoid assembly and starch tethering.

The paper introduces CyanOperon, a toolkit for assembling synthetic operons in the CyanoGate MoClo system, demonstrating its use in biosynthesis pathways and RBS libraries in E. coli and Synechocystis.

This study investigates the role of LCI9 in starch sheath formation and CO2-concentrating mechanism efficiency in Chlamydomonas reinhardtii, finding that LCI9 is essential for proper sheath assembly and pCCM function.

The paper identifies a conserved linker protein from Chlorella that can phase separate Rubisco in Chlamydomonas and some plants, enabling cross-species pyrenoid formation.

This study identifies proteins involved in pyrenoid membrane biogenesis and shows their role in CO2 delivery, with implications for engineering functional pyrenoids in crops.

This study shows that SAGA1 and SAGA2 proteins in Arabidopsis chloroplasts promote the formation of starch structures around proto-pyrenoids, contributing to CO2-concentrating mechanisms.

The paper characterizes genetic tools for Synechococcus sp. PCC 11901, including an inducible promoter system, CRISPRi, and markerless genome editing with Cas12a.

This study identifies the structural basis of Rubisco phase separation in the pyrenoid by analyzing interactions between Rubisco and its linker protein EPYC1 in Chlamydomonas reinhardtii.

The paper introduces CyanoGate, a modular cloning system for cyanobacteria based on the plant MoClo syntax, enabling knock-outs, integrations, and multigene expression.