Satoshi Akanuma

Restricted amino acid diversity alters enzymatic phosphoryl-transfer catalysis

This study shows that restricting amino acid diversity can replace native enzymatic activity with an alternative phosphoryl-transfer reaction in a reconstructed ancestral nucleoside diphosphate kinase.

Functional and Structural Characterization of F1-ATPase with common ancestral core domains in stator ring

The paper characterizes the functional and structural properties of an ancestral F1-ATPase, revealing it was originally a 6-stepper that diversified into 3-, 6-, and 9-step forms.

Insights into the low-temperature adaptation of an enzyme as studied through ancestral sequence reconstruction

This study uses ancestral sequence reconstruction to identify amino acid substitutions responsible for low-temperature adaptation in an enzyme.