Novel reporter of the PINK1-Parkin mitophagy pathway identifies its damage sensor in the import gate
Julia A. Thayer, Jennifer D. Petersen, Xiaoping Huang, Luiza M Gruel Budet, James Hawrot, Daniel M. Ramos, Shiori Sekine, Yan Li, Michael Emmerson Ward, Derek P. Narendra
A HaloTagged MFN2 reporter enabled CRISPRi screens showing diverse mitochondrial damage activates PINK1-Parkin via membrane potential loss, with TOM retaining PINK1 when TIM23 is blocked.
The EMBO Journal · Nov 20, 2025Peer-reviewedCell Biology✦ In-depth digest