Molecular BiologyThe CRISPR JournalPeer-reviewed
Single step, high efficiency CRISPR-Cas9 genome editing in primary human disease-derived fibroblasts
Matteo Martufi, Robert B. Good, Radu Rapiteanu…·Klio Maratou
This study develops a single-step CRISPR-Cas9 method for high-efficiency genome editing in primary human fibroblasts without antibiotic selection or clonal expansion, achieving >90% knockout efficiency.
28 citations