Radu Rapiteanu

Variants in tubule epithelial regulatory elements mediate most heritable differences in human kidney function

This paper identifies genetic variants in tubule epithelial regulatory elements that explain most heritable differences in human kidney function using GWAS, scATAC-seq, and functional assays.

Mapping the functional impact of non-coding regulatory elements in primary T cells through single-cell CRISPR screens

The paper develops a CRISPRi-based single-cell screening approach to map non-coding regulatory elements to genes in primary human CD4+ T cells and demonstrates its utility for GWAS effector gene identification.

Highly efficient genome editing in primary bronchial epithelial cells establishes FOXJ1 as essential for ciliation in human airways

This study uses genome editing to show that FOXJ1 is essential for ciliation in human airway epithelial cells.

Single step, high efficiency CRISPR-Cas9 genome editing in primary human disease-derived fibroblasts

This study develops a single-step CRISPR-Cas9 method for high-efficiency genome editing in primary human fibroblasts without antibiotic selection or clonal expansion, achieving >90% knockout efficiency.