Single step, high efficiency CRISPR-Cas9 genome editing in primary human disease-derived fibroblasts
Matteo Martufi, Robert B. Good, Radu Rapiteanu, Tobias Schmidt, Eleni Patili, Ketil Tvermosegaard, Maria I. New, Carmel B. Nanthakumar, Joanna C. Betts, Andy D. Blanchard, Klio Maratou
This study develops a single-step CRISPR-Cas9 method for high-efficiency genome editing in primary human fibroblasts without antibiotic selection or clonal expansion, achieving >90% knockout efficiency.
The CRISPR Journal · Feb 1, 2019Peer-reviewedMolecular Biology