Penalised regression improves imputation of cell-type specific expression using RNA-seq data from mixed cell populations compared to domain-specific methods
Wei‐Yu Lin, Melissa Kartawinata, Bethany R. Jebson, Restuadi Restuadi, Hannah Peckham, Anna Radziszewska, Claire T. Deakin, Coziana Ciurtin, Lucy R. Wedderburn, Chris Wallace
This paper compares methods for imputing cell-type specific expression from mixed RNA-seq data, finding that machine learning methods like LASSO and ridge outperform domain-specific methods in recovering differential gene expression.
PLoS Computational Biology · Mar 7, 2025Peer-reviewedBioinformatics