Optimization of Cas12a for multiplexed genome-scale transcriptional activation
Audrey L Griffith, Fengyi Zheng, Abby V. McGee, Nathan Miller, Zsofia M Szegletes, Ganna Reint, Fabian Gademann, Ifunanya Nwolah, Mudra Hegde, Yanjing V. Liu, Amy B. Goodale, John G. Doench
The paper optimizes Cas12a for multiplexed CRISPRa screens in human cells, enabling simultaneous activation of up to four genes and identifying criteria for selecting effective guides.
Cell Genomics · Sep 1, 2023Peer-reviewedGenomics