Author

Fengyi Zheng

Broad InstituteORCID 0000-0003-4479-604XUnclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Assay:CRISPR screen· 2 Organism:human· 2 Research Focus:perturbation response prediction· 2 Approach:deep learning· 1 Research Focus:drug response / drug discovery· 1 Approach:experimental assay· 1 Approach:statistical model· 1

Frequent Co-Authors

Ganna ReintiD· 2 shared John G. DoenchiD· 2 shared Abby V. McGeeiD· 1 shared Allison Uebele· 1 shared Amy B. GoodaleiD· 1 shared Audrey L GriffithiD· 1 shared Daytan GibsoniD· 1 shared E L KaplaniD· 1 shared

Activity by Year

2023-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 29 mutual co-author links

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2 shared papersBioengineering

Ganna ReintORCID

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2 papers

Recent·Most cited

BioengineeringbioRxivPreprintFeb 26, 2026

Optimized parameters for Cas9 CRISPR interference library design

Smriti Srikanth, Fengyi Zheng, Laura M. Drepanos…·John G. Doench

This study designs an optimized Cas9 CRISPRi library by improving on-target scoring and analyzing off-target effects using updated datasets and methods.

2 citations

humanCRISPR screen

𝕏 PostBluesky

GenomicsCell GenomicsPeer-reviewedSep 1, 2023

Optimization of Cas12a for multiplexed genome-scale transcriptional activation

Audrey L Griffith, Fengyi Zheng, Abby V. McGee…·John G. Doench

The paper optimizes Cas12a for multiplexed CRISPRa screens in human cells, enabling simultaneous activation of up to four genes and identifying criteria for selecting effective guides.

29 citations

humanCRISPR screen

𝕏 PostBluesky