Yanjing V. Liu

Multi-locus CRISPRi targeting with a single truncated guide RNA

This paper develops a CRISPRi approach using truncated guides to screen multiple transcription factor binding sites, showing they can disrupt enhancer activity and repress transcription factor binding.

Modular vector assembly enables rapid assessment of emerging CRISPR technologies

The paper introduces a modular CRISPR vector assembly system called Fragmid, enabling rapid creation and testing of CRISPR technologies through combinatorial design and pooled screening.

Optimization of Cas12a for multiplexed genome-scale transcriptional activation

The paper optimizes Cas12a for multiplexed CRISPRa screens in human cells, enabling simultaneous activation of up to four genes and identifying criteria for selecting effective guides.

Formation and recycling of an active epigenetic mark mediated by cell cycle-specific RNAs

The paper shows that cell cycle-specific long noncoding RNAs regulate the establishment and recycling of the acetylated histone H2A.Z epigenetic mark.

Targeted intragenic demethylation initiates chromatin rewiring for gene activation

CRISPR-DiR induces locus-specific demethylation and chromatin remodeling to reactivate the p16 gene.