М. Л. Филипенко

The first poly(A) polymerase from Alphaproteobacteria

This study characterizes the first poly(A) polymerase from Alphaproteobacteria, revealing its biochemical properties and contrasting behavior with E. coli PAP-1.

Insights into the performance of CusF as a solubility tag for recombinant protein expression

This study evaluates the solubility-enhancing and purification capabilities of the CusF tag for two recombinant proteins, finding it ineffective for both.

Comparison of biochemical properties of RNases HII from E. coli, Geobacillus stearothermophilus and Thermus thermophilus

This study compares the biochemical properties of RNase HII from three organisms, identifying thermal stability and cofactor preferences to guide practical applications.

eLaRodON: identification of large genomic rearrangements in Oxford Nanopore sequencing data

eLaRodON improves large genomic rearrangement detection in Oxford Nanopore data, outperforming existing tools with higher accuracy across multiple datasets.

Optimization of conditions for production of soluble E. coli polyA-polymerase

This study optimizes conditions for producing soluble E. coli PAP-1, identifying BL21 (DE3) pLysS as the best strain for yield, solubility, and activity.

A novel approach for simultaneous detection of structural and single-nucleotide variants based on a combination of chromosome conformation capture and exome sequencing

This paper introduces a method combining exome sequencing and chromosome conformation capture to detect both SNVs and SVs, demonstrating its effectiveness in identifying causative variants in rare disorders.

A novel thermostable and processive reverse transcriptase from a group II intron of Anoxybacillus flavithermus

The paper characterizes a novel thermostable and processive reverse transcriptase from Anoxybacillus flavithermus, showing its potential for applications requiring heat-resistant RTs.

Expanding the list of sequence-agnostic enzymes for chromatin conformation capture assays with S1 nuclease

This paper introduces an S1 nuclease-based Hi-C method for chromatin conformation capture, offering improved chromatin fragmentation and library preparation compared to DNase I Hi-C.

A spectrum of BRCA1 and BRCA2 germline deleterious variants in ovarian cancer in Russia

The paper analyzes BRCA1 and BRCA2 mutations in ovarian cancer patients from Russia, identifying common and novel variants and estimating the age of a founder mutation.

NGS-PrimerPlex: high-throughput primer design for multiplex polymerase chain reactions

NGS-PrimerPlex designs primers for multiplex PCR to create NGS panels, achieving high coverage in sequencing the LRRK2 gene.

The attachment of Sso7d-like protein improves processivity and resistance to inhibitors of M-MuLV reverse transcriptase

This study creates chimeric reverse transcriptases by fusing M-MuLV RT with DNA-binding domains, improving processivity and inhibitor resistance.