Hiroshi Shimizu

This study evaluates the precision of 13C metabolic flux analysis using different glucose tracers to estimate non-oxidative glycolysis fluxes in E. coli.

This study characterizes central carbon metabolism in Acinetobacter sp. Tol 5 using 13C-MFA, revealing flux patterns under different carbon sources and metabolic pathways.

OpenMebius2 is a GUI-based software for 13C-metabolic flux analysis that suggests tracer labeling patterns to improve flux prediction accuracy.

The paper constructs a combinatorial repression library in E. coli to optimize flux distribution for mevalonate production by modifying ribosome-binding sites of key genes.

This paper introduces a method to predict enzyme substrate specificity residues using homologous sequence information and validates it with experimental tests on enzyme pairs.