dFLASH; dual FLuorescent transcription factor Activity Sensor for Histone integrated live-cell reporting and high-content screening
Timothy P. Allen, Alison E. Roennfeldt, Moganalaxmi Reckdharajkumar, Adrienne E. Sullivan, Miaomiao Liu, Ronald J. Quinn, Darryl Lyndon Russell, Daniel J. Peet, Murray L. Whitelaw, David C. Bersten
The paper introduces dFLASH, a tool for live-cell reporting of transcription factor activity, demonstrating its use in sensing HIF and PGR activities and screening for HIF pathway modulators.
Nature Communications · Apr 7, 2025Peer-reviewedSynthetic Biology