Sensitive detection of protein ubiquitylation using a protein-fragment complementation assay
Marie Le Boulch, Audrey Brossard, Gaëlle Le Dez, Sébastien Léon A, Gwénaël E. E. Rabut
The paper introduces NUbiCA, a sensitive protein-fragment complementation assay for detecting ubiquitylation events in cultured cells and model organisms, demonstrating its application to study Cse4 ubiquitylation during S-phase.
Journal of Cell Science · May 14, 2020Peer-reviewedMolecular Biology