Gwénaël E. E. Rabut

HInt uses AlphaFold to predict protein interactions and identifies homologous proteins by conserved interaction interfaces, revealing previously unknown relationships in prokaryotic and eukaryotic systems.

The paper introduces NUbiCA, a sensitive protein-fragment complementation assay for detecting ubiquitylation events in cultured cells and model organisms, demonstrating its application to study Cse4 ubiquitylation during S-phase.