Author

Sean M. Hughes

University of WashingtonORCID 0000-0002-9409-9405Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Organism:human· 4 Research Focus:cell type annotation· 3 Approach:experimental assay· 3 Research Focus:cell-cell communication· 1 Research Focus:drug response / drug discovery· 1 Assay:flow cytometry· 1 Assay:metagenomics· 1 Organism:mouse· 1

Frequent Co-Authors

Florian HladikiD· 4 shared Claire LevyiD· 2 shared Jennifer M. LundiD· 2 shared Martin PrliciD· 2 shared Adam BurgeneriD· 1 shared Alana LamontiD· 1 shared Alicia R. BerardiD· 1 shared Anja Germann· 1 shared

Activity by Year

2016-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 21 mutual co-author links

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4 shared papersImmunology

Florian HladikORCID

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Author profile →

4 papers

Recent·Most cited

Menstruation is associated with cyclical granulysin peaks in vaginal secretions despite stable expression by cervicovaginal immune cells

Sean M. Hughes, Claire Levy, Dendron R Chamberlain, Dana Varon, Britt Murphy, Katharine V. Schwedhelm, Juma Shafi, Jennifer M. Lund, Martin Prlic, Stephen C. De Rosa, Elizabeth Micks, Christine Johnston, Florian Hladik

The paper shows granulysin levels in vaginal secretions peak during menstruation but not the luteal phase, linked to increased granulysin-expressing cells in the endocervical canal.

American Journal of Reproductive Immunology · Aug 1, 2026Peer-reviewedImmunology

Computational Microbiome Pharmacology Analysis Elucidates the Anti-Cancer Potential of Vaginal Microbes and Metabolites

Damilola C. Lawore, Smrutiti Jena, Alicia R. Berard, Kenzie Birse, Alana Lamont, Romel D. Mackelprang, Laura Noël‐Romas, Michelle Perner, Xuanlin Hou, Elizabeth Irungu, Nelly Mugo, Samantha Knodel, Timothy R. Muwonge, Elly Katabira, Sean M. Hughes, , Douglas K. Brubaker

The paper develops a systems biology framework called the Pharmacobiome to analyze the anti-cancer potential of vaginal microbes and metabolites by comparing their effects on host gene expression to known drugs.

Frontiers in Microbiology · Sep 16, 2025Peer-reviewedSystems Biology

Mucosal tissue NK cells directly mediate tissue protections and repair during infection

Sarah C. Vick, Eva Domenjo‐Vila, Marie Frutoso, Raisa A. Glabman, Lakshmi S. Warrier, Sean M. Hughes, Anna C. Kirby, Michael F. Fialkow, Florian Hladik, Martin Prlic, Jennifer M. Lund

This study identifies a reparative role for mucosal NK cells in tissue protection and repair during infection, revealing their dual function in immunity and tissue homeostasis.

bioRxiv · Apr 4, 2025 · PreprintImmunology

Cryopreservation of human mucosal leukocytes

Sean M. Hughes, Zhiquan Shu, Claire N. Levy, April L. Ferre, Heather Hartig, Cifeng Fang, Gretchen Lentz, Michael Fialkow, Anna C Kirby, Kristina M Adams Waldorf, Ronald S Veazey, Anja Germann, Hagen von Briesen, M. Juliana McElrath, Charlene S Dezzutti, , Florian Hladik

This study identifies effective cryopreservation media and protocols for mucosal leukocytes, achieving over 75% recovery and providing a detailed protocol for their preservation.

PLOS ONE · May 27, 2016Peer-reviewedImmunology