Jonas De Saeger

The paper introduces B3, a modular Golden Gate system for assembling Agrobacterium binary vector backbones, enabling efficient and flexible construction of over 370,000 possible constructs.

This paper introduces a method called oligo stitching that enhances Golden Gate cloning by using single-stranded DNA oligos to convert parts between standards and assemble incompatible parts without PCR.

The paper develops an optimized LbCas12a-ABE base editor in wheat and maize using the ITER platform, achieving 40% activity on a GFP reporter through iterative design.