Tobias B. Alter

Protein without farms: What comparative genomics reveals about ''Power-to-Food'' microbes

This paper compares two hydrogen-oxidizing bacteria strains to identify genomic features that inform the design of safe, efficient food-grade microbes for power-to-protein production.

Growth-coupled microbial biosynthesis of the animal pigment xanthommatin

This paper introduces a growth-coupled biosynthesis strategy (GrowBio) for producing xanthommatin, demonstrating its effectiveness in microbial production through engineered Pseudomonas putida.

Metabolic growth coupling strategies for in vivo enzyme selection systems

The paper presents a computational workflow to design enzyme selection systems (ESSs) that couple enzyme activity to microbial metabolism, creating a database of 25,505 ESS designs for Escherichia coli.

Protein allocation and enzymatic constraints explain Escherichia coli wildtype and mutant phenotypes

This study develops a protein allocation model to predict Escherichia coli wildtype and mutant phenotypes by integrating protein distribution with metabolic fluxes.

High titer methyl ketone production with tailored Pseudomonas taiwanensis VLB120

This paper engineers Pseudomonas taiwanensis VLB120 to produce methyl ketones with a 4-fold higher titer than previous strains, achieving 9.8 g L-1.

Determination of growth-coupling strategies and their underlying principles

The paper develops a computational tool, gcOpt, to identify growth-coupling strategies that maximize product yield by enforcing strict coupling between product synthesis and microbial growth.