Pascal A. Pieters

The paper presents a computational workflow to design enzyme selection systems (ESSs) that couple enzyme activity to microbial metabolism, creating a database of 25,505 ESS designs for Escherichia coli.

PanKB is a microbial pangenome knowledgebase that integrates pangenomic data and literature to support research and biotechnological applications.

The paper characterizes a synthetic coherent feed-forward loop (CFFL) in E. coli cell-free systems, showing it can repress background activity but is not effective as a temporal filter.

The paper develops DNA-encoded sender-receiver architectures using microfluidic trapping arrays and light-activated DNA strand displacement to study diffusion-based signaling and spatial integration of chemical communication.