Hualan Liu

This study uses random barcode transposon-site sequencing in Mycobacterium tuberculosis to identify gene functions, revealing novel phenotypes and metabolic pathways.

The paper identifies and validates novel plasmid origins of replication from an environmental Pantoea strain, enabling expression of heterologous proteins and pathways in both model and non-model bacteria.

The paper introduces a method called 'magic pools' to efficiently assess transposon delivery vectors in bacteria by using barcoded libraries and sequencing to identify effective vectors for mutagenesis.