Yuan Cai

MERFISH 2.0 improves transcript detection sensitivity in degraded and archival tissues, enhancing spatial transcriptomic analysis of human and mouse samples.

The paper introduces Hip-seq, a method to reduce contamination in single-cell capture, and finds that axon regeneration failure is linked to translation and circadian clock abnormalities.

This study uses CRISPR to knockout RHO in rhesus macaques, resulting in RP-like phenotypes and demonstrating the feasibility of creating NHP models for retinal disease research.