Synthetic BiologyACS Synthetic BiologyPeer-reviewed
Differentially optimized cell-free buffer enables robust expression from unprotected linear DNA in exonuclease-deficient extracts
Angelo Cardoso Batista, Antoine Lévrier, Paul Soudier…·Manish Kushwaha
This paper develops exonuclease-deficient cell-free extracts and optimized buffers to enable robust expression from unprotected linear DNA without additional protection strategies.
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