Antoine Lévrier

A synthetic cell phage cycle

This study creates a synthetic cell-free system where T7 phages infect synthetic cells, demonstrating a complete viral life cycle in vitro.

Split minireporters facilitate monitoring of gene expression and peptide production in linear cell-free transcription-translation systems

This paper introduces minireporters for monitoring gene expression and peptide production in cell-free systems, reducing DNA length and synthesis costs while maintaining reliable output.

PHEIGES, all-cell-free phage synthesis and selection from engineered genomes

The paper introduces PHEIGES, a method to rapidly engineer and select phages using cell-free transcription-translation, producing high titers of engineered phages within one day.

Differentially optimized cell-free buffer enables robust expression from unprotected linear DNA in exonuclease-deficient extracts

This paper develops exonuclease-deficient cell-free extracts and optimized buffers to enable robust expression from unprotected linear DNA without additional protection strategies.