Yuki Hata

This study generates KAI2 loss-of-function mutants in the fern Ceratopteris richardii using a CRISPR/Cas9 system and shows KAI2-dependent signaling is conserved.

The paper uses snRNA-seq to analyze moss stem cells, identifying a cytokinin-ESR module that promotes pluripotent stem cell identity in the gametophore apical cell.

This study identifies KAI2-dependent signaling as a regulator of vegetative reproduction in Marchantia polymorpha by activating MpLOG-mediated cytokinin synthesis, which in turn controls GCAM1 expression.

The paper shows cytokinins are confined to pluripotent stem cells in moss, excluding the differentiation factor TAW1 to establish the shoot apical meristem from a single stem cell.