Douglas R. Brumley

This paper develops an expansion microscopy workflow to visualize subcellular features of Breviolum minutum, a cnidarian photosymbiont, overcoming challenges posed by autofluorescence and small size.

This study applies FastSAM for automated algal cell segmentation and clustering in water samples, showing high accuracy for single cells and varying clustering performance.

The paper uses fluorescence lifetime imaging microscopy to study the spatial association between B. minutum and intracellular bacteria under light-dark cycles, finding changes in bacterial abundance linked to photoperiod.