Patrick S. Donahue

This study enhances synthetic hypoxia biosensors by integrating natural hypoxia-responsive factors and feedback circuits to improve their performance and understanding of hypoxia regulation.

This paper presents a method for designing mammalian genetic programs using predictive models and modular genetic parts to achieve complex cellular functions.

COMET provides a toolkit of transcription factors and promoters to design and tune gene expression in mammalian cells with customizable genetic programs.

This study improves synthetic receptor performance by showing that transmembrane domains significantly affect signaling through intracellular geometry, and proposes design principles for future receptors.