Joseph J. Muldoon

This paper develops a non-viral BCMA CAR-T therapy using CRISPR and 1XX signaling, showing improved safety, potency, and persistence in multiple myeloma.

Combining TCE therapy with IL-2 enhances TCE efficacy in aggressive small-cell lung cancer and melanoma models with low target antigen density through T cell clonal replacement and CD8+ effector T cell differentiation.

This paper develops a genome engineering workflow for primary human NK cells using CRISPR/Cas9 and AAV6 to integrate and disrupt genes, enhancing anti-tumor function through targeted CAR expression.

This study enhances synthetic hypoxia biosensors by integrating natural hypoxia-responsive factors and feedback circuits to improve their performance and understanding of hypoxia regulation.

This paper introduces PERC, a method for efficient CRISPR editing in primary human immune cells and HSPCs using a peptide reagent, achieving over 90% editing efficiency without specialized equipment.

The paper develops and optimizes a one-pot NASBA-Cas13a assay for rapid, sensitive detection of SARS-CoV-2 RNA without specialized equipment.

This paper develops a method to efficiently reprogram T cells at multiple loci using SEED-Selection, achieving high purity for single and combined gene edits.

The paper introduces GAMES, a workflow for developing dynamic models of synthetic genetic systems, demonstrating it with a case study on a chemically responsive transcription factor.

This paper presents a method for designing mammalian genetic programs using predictive models and modular genetic parts to achieve complex cellular functions.

The paper shows macrophages use quorum licensing, a density-dependent mechanism, to regulate collective activation in response to LPS, leading to bimodal phenotypic partitioning.

COMET provides a toolkit of transcription factors and promoters to design and tune gene expression in mammalian cells with customizable genetic programs.

This study improves synthetic receptor performance by showing that transmembrane domains significantly affect signaling through intracellular geometry, and proposes design principles for future receptors.