Synthetic BiologySynthetic BiologyPeer-reviewed
Integration-coupled activation of promoterless combinatorial pathway libraries in Clostridium avoids burden during DNA assembly
Paweł M. Mordaka, James J. Williamson, John T. Heap
The paper develops a method to enable combinatorial DNA assembly in Clostridium by preventing premature expression during library construction and activating it later through genomic integration.
DOI 10.1093/synbio/ysag008