John T. Heap

The paper develops a method to enable combinatorial DNA assembly in Clostridium by preventing premature expression during library construction and activating it later through genomic integration.

The paper demonstrates a combinatorial metabolic engineering approach in cyanobacteria that achieves stable overproduction of lycopene from CO2 without iterative optimization.

The paper identifies strong transcriptional terminators in Synechocystis sp. PCC 6803 that improve inducible control of chromosomally integrated genetic constructs.

The paper introduces Start-Stop Assembly, a modular DNA assembly method that avoids scar sequences and enables combinatorial assembly for metabolic engineering.

This paper designs and builds two synthetic negative feedback circuits using engineered small RNAs, showing they can fine-tune output and reduce noise compared to traditional protein-based systems.

This paper introduces a rhamnose-inducible promoter system in cyanobacteria with improved properties over existing systems.

The paper identifies the stringency of promoter sequences in Clostridium and shows that adjusting mutation rates in promoter library synthesis improves functionality in this organism.