Paweł M. Mordaka

This paper demonstrates codon compression of the Chlamydomonas chloroplast genome to 51 codons without affecting chloroplast function or photosynthetic growth.

The paper develops a method to enable combinatorial DNA assembly in Clostridium by preventing premature expression during library construction and activating it later through genomic integration.

The paper tests different terminator elements in Chlamydomonas reinhardtii to identify those that maximize transgene expression using a standardized synthetic biology approach.

The paper introduces Start-Stop Assembly, a modular DNA assembly method that avoids scar sequences and enables combinatorial assembly for metabolic engineering.

The paper identifies the stringency of promoter sequences in Clostridium and shows that adjusting mutation rates in promoter library synthesis improves functionality in this organism.