Margaux Aubel

DeNoFo provides a standardised format and toolkit for reproducible de novo gene annotation, enabling consistent documentation and comparison across studies.

The paper produces gapless assemblies of ape sex chromosomes for five great apes and a lesser ape, revealing divergence in Y chromosomes and evolutionary dynamics.

This study improves the expression of de novo proteins in E. coli by using chaperones and specific expression systems, increasing solubility from 30% to 62%.