Molecular BiologybioRxivPreprint
Drosophila cap-binding protein eiF4EHP promotes translation via a 3'UTR-dependent mechanism under hypoxia and contributes to fruit fly adaptation to oxygen variations
DOI 10.1101/2022.07.20.500870
Author
Université Libre de BruxellesORCID 0000-0003-3097-7667Unclaimed ORCID profile · follow to get notified of new preprints
2 papers
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Claude Van Campenhout, Pauline Cabochette, Anne-Clémence Veillard…·Véronique Kruys
The paper provides guidelines and tools to optimize CRISPR/Cas9 genome targeting efficiency and specificity by considering target locus, guide RNA design, delivery methods, and off-target detection.