Molecular BiologyJournal of Bioinformatics and Systems BiologyPeer-reviewed
A new method and application of PacBio sequencing for low copy and difficulty preparation plasmids.
Xiaoshu Ma, Yusha Wang, Ruikai Jia, Hua Ye
This paper presents a method to improve PacBio sequencing of low-copy plasmids using Phi29 polymerase amplification and a plasmid-free sequencing approach.