Cas9AEY (Cas9-facilitated Homologous Recombination Assembly of non-specific Escherichia coli yeast vector) method of constructing large-sized DNA.
The paper describes a method to construct large DNA plasmids using yeast homologous recombination and CRISPR-Cas9 to remove a yeast replication element.
bioRxiv · Sep 17, 2024 · PreprintSynthetic Biology