Ryan M. Ziels

The paper uses activity-targeted metaproteomics to study rare syntrophic bacteria in anaerobic communities, revealing their metabolic activity and a previously hypothesized oxidative glycine pathway.

PUPpy designs taxon-specific primers for substrain-level microbial detection and absolute quantification, outperforming 16S rRNA and shotgun sequencing in defined communities.

Dual patterned unique molecular identifiers (UMIs) turn Nanopore or PacBio CCS reads of ~4400 bp rRNA operons into single-molecule consensus sequences with 0.0049% (ONT) or 0.0006% (PacBio) mean error.