Jessica T. Stieglitz

The paper identifies yeast gene knockouts that enhance noncanonical amino acid incorporation efficiency at amber stop codons using a pooled knockout screen and FACS.

This paper reports the discovery of new aminoacyl-tRNA synthetase variants in yeast for genetic code expansion, doubling the number of available translationally active aaRSs.

This paper demonstrates that the Methanomethylophilus alvus PylRS and its tRNA can incorporate noncanonical amino acids into yeast proteins, expanding the toolkit for genetic encoding in yeast.

The paper introduces single plasmid systems for genetic code expansion in yeast and shows they are as effective as dual plasmid systems for noncanonical amino acid incorporation.

This paper evaluates how different reporter systems affect measurements of noncanonical amino acid incorporation in yeast, finding that dual-fluorescent protein reporters provide consistent and precise quantification.