GenomicsNucleic Acids ResearchPeer-reviewed
Specificity of RNAi, LNA and CRISPRi as loss-of-function methods in transcriptional analysis
Lovorka Stojic, Aaron T. L. Lun, Jasmin Mangei…·Duncan T. Odom
This study compares the off-target effects of RNAi, antisense oligonucleotides, and CRISPRi in transcriptomic analyses, showing that all methods have non-negligible off-target effects and recommend using multiple LOF methods for functional validation.
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