Specificity of RNAi, LNA and CRISPRi as loss-of-function methods in transcriptional analysis
Lovorka Stojic, Aaron T. L. Lun, Jasmin Mangei, Patrice Mascalchi, Valentina Quarantotti, Alexis R. Barr, Chris Bakal, John C. Marioni, Fanni V. Gergely, Duncan T. Odom
This study compares the off-target effects of RNAi, antisense oligonucleotides, and CRISPRi in transcriptomic analyses, showing that all methods have non-negligible off-target effects and recommend using multiple LOF methods for functional validation.
Nucleic Acids Research · May 9, 2018Peer-reviewedGenomics