Ryozo Imai

The paper combines CRISPR-Cas9 RNP delivery via particle bombardment with leaf regeneration to achieve non-chimeric genome editing in apple without DNA integration.

The paper develops a DNA-free CRISPR/Cas9 RNP-based genome editing method in melon to create a long shelf-life mutant by targeting CmACO1, reducing ethylene during ripening.

This paper develops a DNA-free CRISPR/Cas9 system in soybean using iPB-RNP method, achieving genome editing in shoot apical meristems without tissue culture, resulting in 0.4-4.6% mutation transmission to next generation.