Caroline Hiefinger

This study enhances the reversible photocontrol of a photoxenase by engineering conformational shifts, achieving a light-regulation factor of ~100 with near-quantitative reversibility.

This study designs and synthesizes six new photoswitchable unnatural amino acids (psUAAs) for enzyme engineering, showing enhanced interaction potential and diverse photochemical behaviors.

The paper demonstrates a new method for reversible enzyme regulation using dichromatic irradiation of azobenzene, showing a two-fold increase in catalytic activity for one enzyme variant.