Nicholas Hassan

This study characterizes PUB33, an Arabidopsis RLCK-IXb/PUB-VI protein, showing it has dual kinase and E3 ubiquitin ligase activities regulated by autophosphorylation and interacts with the bacterial effector RipV1 to modulate immune responses.

This paper optimizes BONCAT for low-disruption labeling of Arabidopsis proteins, showing improved growth and effective enrichment under stress conditions.