BioengineeringbioRxivPreprint
FRAGMENT MORPHOMETRY ANALYSIS AND SAME-COLOR-CHANNEL SEPARATION ENABLE OBJECTIVE QUANTIFICATION ACROSS BBB MODELS
Benjamin D Peck, Nicholas R. O’Hare, Craig F. Ferris…·Eno Essien Ebong
The paper introduces two image-analysis pipelines to objectively quantify BBB integrity in vitro and in vivo, showing reduced ZO-1 fragment area and fragmentation ratio in glycocalyx knockdown models and altered markers in aged and Alzheimer's mice.